Aberrant guanosine triphosphate-beta-tubulin interaction in Alzheimer's disease.

@article{Khatoon1989AberrantGT,
  title={Aberrant guanosine triphosphate-beta-tubulin interaction in Alzheimer's disease.},
  author={Sayadda Khatoon and Shannon R. Campbell and Boyd E. Haley and John T. Slevin},
  journal={Annals of neurology},
  year={1989},
  volume={26 2},
  pages={210-5}
}
Guanosine triphosphate (GTP) is an absolute requirement for tubulin polymerization in situ. The nucleotide photoaffinity probe 8-azidoguanosine 5'-triphosphate (8N3GTP) has been shown to be a biological mimic of GTP in this system and, also, an effective active site probe of the exchangeable GTP binding site. Using [32P]8N3GTP we demonstrate that the exchangeable GTP site of the beta subunit of tubulin is available to added guanine nucleotide in normal aged brain homogenates, whereas it is… CONTINUE READING