Aberrant guanosine triphosphate–beta‐tubulin interaction in Alzheimer's disease

  title={Aberrant guanosine triphosphate–beta‐tubulin interaction in Alzheimer's disease},
  author={S. Khatoon and S. Campbell and B. Haley and J. Slevin},
  journal={Annals of Neurology},
Guanosine triphosphate (GTP) is an absolute requirement for tubulin polymerization in situ. The nucleotide photoaffinity probe 8‐azidoguanosine 5′‐triphosphate (8N3GTP) has been shown to be a biological mimic of GRP in this system and, also, an effective active site probe of the exchangeable GRP binding site. Using [32P]8N3GTP we demonstrate that the exchangeable GTP site of the beta subunit of tubulin is available to added guanine nucleotide in normal aged brain homogenates, whereas it is… Expand
Tau binds ATP and induces its aggregation
Identification of the guanine binding domain peptide of the GTP‐binding site of glucagon
Site-specific photobiotinylation of immunoglobulins, fragments and light chain dimers.
Nucleotide photoaffinity labeling of protein kinase subunits.
  • B. Haley
  • Chemistry, Medicine
  • Methods in enzymology
  • 1991