Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error

  title={Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error},
  author={Hong-Rye Kim and Jae Eun Lee and Reza K. Oqani and So Yeon Kim and Teruhiko Wakayama and Chong Li and Su Jin Sa and Je Seok Woo and Dong Il Jin},
  journal={PLoS ONE},
Cloned mice derived from somatic or ES cells show placental overgrowth (placentomegaly) at term. We had previously analyzed cloned and normal mouse placentae by using two-dimensional gel electrophoresis and mass spectrometry to identify differential protein expression patterns. Cloned placentae showed upregulation of tissue inhibitor of metalloproteinase-2 (TIMP-2), which is involved in extracellular matrix degradation and tissue remodeling, and downregulation of pre-B cell colony enhancing… Expand
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Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell.
Placentomegaly is a common phenotype in cloned mice. To assess differences in protein expression between placentae of cloned and uncloned mice, we used a proteomic approach involving 2-dimensionalExpand
Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae.
Western blot analysis suggests that key proteins of placental development are abnormally expressed in SCNT cloned bovine placentae, probably resulting in abnormal placental function and clonal mortality. Expand
Epigenetic characterization of the PBEF and TIMP-2 genes in the developing placentae of normal mice.
It is suggested that epigenetic changes may regulate gene expression level in the developing placentae of normal mice and that inappropriate epigenetic reprogramming might be one cause of the abnormal placenta seen in cloned animals. Expand
Expression and function of matrix metalloproteinases and their inhibitors at the maternal-embryonic boundary during mouse embryo implantation.
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The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice
It is concluded that the Sall3 locus is the area with frequent epigenetic errors in cloned mice, suggesting that there exists at least genetic locus that is highly susceptible to epigenetic error caused by nuclear transfer. Expand
Pre-B-cell colony-enhancing factor, a novel cytokine of human fetal membranes.
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Variation in Gene Expression and Aberrantly Regulated Chromosome Regions in Cloned Mice1
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Expression and activity of matrix metalloproteinase 2 and 9 in human trophoblasts.
Invasive ability of early CTs was inhibited by tissue inhibitor of metalloproteinase (TIMP)-2 and MMP-2 antibody in a dose dependent manner, suggesting that the invasive ability of trophoblasts may be regulated by the enzyme activity of gelatinases, especially M MP-2. Expand
The effects of pre-B-cell colony-enhancing factor on the human fetal membranes by microarray analysis.
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Gene expression patterns in bovine in vitro-produced and nuclear transfer-derived embryos and their implications for early development.
Several aberrations were found in IVP and NT-derived embryos, including a complete lack of expression, an induced expression, or a significant up- or downregulation of a specific gene, hypothesize that the alterations are caused by epigenetic modifications, primarily by changes in the methylation patterns. Expand