Abbreviated incubation times for human prions in mice expressing a chimeric mouse-human prion protein transgene.

@article{Korth2003AbbreviatedIT,
  title={Abbreviated incubation times for human prions in mice expressing a chimeric mouse-human prion protein transgene.},
  author={Carsten Korth and Kiyotoshi Kaneko and Darlene Groth and Norbert Heye and Glenn Telling and James Mastrianni and Piero Parchi and Pierluigi Gambetti and Robert Will and James Ironside and Cornelia Heinrich and Patrick Tremblay and Stephen J. DeArmond and Stanley B Prusiner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 8},
  pages={4784-9}
}
Transgenic (Tg) mouse lines that express chimeric mouse-human prion protein (PrP), designated MHu2M, are susceptible to prions from patients with sporadic Creutzfeldt-Jakob disease (sCJD). With the aim of decreasing the incubation time to fewer than 200 days, we constructed transgenes in which one or more of the nine human residues in MHu2M were changed to mouse. The construct with murine residues at positions 165 and 167 was expressed in Tg(MHu2M,M165V,E167Q) mice and resulted in shortening… CONTINUE READING