Abbreviated Pathway for Biosynthesis of 2-Thiouridine in Bacillus subtilis.

@article{Black2015AbbreviatedPF,
  title={Abbreviated Pathway for Biosynthesis of 2-Thiouridine in Bacillus subtilis.},
  author={Katherine A. Black and Patricia C Dos Santos},
  journal={Journal of bacteriology},
  year={2015},
  volume={197 11},
  pages={1952-62}
}
UNLABELLED The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize the anticodon structure, improving ribosomal binding and overall efficiency of the translational process. Biosynthesis of s(2)U in Escherichia coli requires a cysteine desulfurase (IscS), a thiouridylase (MnmA), and five intermediate sulfur-relay enzymes (TusABCDE). The E. coli MnmA adenylates and subsequently thiolates tRNA to form the s(2)U… CONTINUE READING