Abasic site binding by the human apurinic endonuclease, Ape, and determination of the DNA contact sites.

@article{Wilson1997AbasicSB,
  title={Abasic site binding by the human apurinic endonuclease, Ape, and determination of the DNA contact sites.},
  author={David M. Wilson and Masaru Takeshita and Bruce Demple},
  journal={Nucleic acids research},
  year={1997},
  volume={25 5},
  pages={
          933-9
        }
}
The mutagenic and lethal effects of abasic sites in DNA are averted by repair initiated by 'class II' apurinic (AP) endonucleases, which cleave immediately 5'to abasic sites. We examined substrate binding by the human AP endonuclease, Ape protein (also called Hap1, Apex or Ref-1). In electrophoretic mobility-shift experiments, Ape bound synthetic DNA substrates containing single AP sites or tetrahydrofuran (F) residues. No complexes were detected with single-stranded substrates or unmodified… CONTINUE READING
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