AU Binding Proteins Recruit the Exosome to Degrade ARE-Containing mRNAs

  title={AU Binding Proteins Recruit the Exosome to Degrade ARE-Containing mRNAs},
  author={Ching-Yi Chen and R. Gherzi and S. Ong and E. Chan and R. Raijmakers and G. Pruijn and G. Stoecklin and C. Moroni and M. Mann and M. Karin},
  • Ching-Yi Chen, R. Gherzi, +7 authors M. Karin
  • Published 2001
  • Biology, Medicine
  • Cell
  • Inherently unstable mammalian mRNAs contain AU-rich elements (AREs) within their 3' untranslated regions. Although found 15 years ago, the mechanism by which AREs dictate rapid mRNA decay is not clear. In yeast, 3'-to-5' mRNA degradation is mediated by the exosome, a multisubunit particle. We have purified and characterized the human exosome by mass spectrometry and found its composition to be similar to its yeast counterpart. Using a cell-free RNA decay system, we demonstrate that the… CONTINUE READING

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    Publications referenced by this paper.
    AU-rich elements: characterization and importance in mRNA degradation.
    • 1,884
    mRNA stability in mammalian cells.
    • 1,165
    • PDF
    Functions of the exosome in rRNA, snoRNA and snRNA synthesis
    • 543
    • Highly Influential
    • PDF
    Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay.
    • 364
    • Highly Influential
    • PDF
    Purification, characterization, and cDNA cloning of an AU-rich element RNA-binding protein, AUF1.
    • 552
    • PDF