AU‐rich RNA‐binding induces changes in the quaternary structure of AUH

  title={AU‐rich RNA‐binding induces changes in the quaternary structure of AUH},
  author={Kazuki Kurimoto and Kanako Kuwasako and Alan M. Sandercock and Satoru Unzai and Carol V. Robinson and Yutaka Muto and Shigeyuki Yokoyama},
  journal={Proteins: Structure},
The human AU RNA binding protein/enoyl‐Coenzyme A hydratase (AUH) is a 3‐hydroxy‐3‐methylglutaconyl‐CoA dehydratase in the leucine degradation pathway. It also possesses an RNA‐binding activity to AUUU repeats, which involves no known conserved RNA‐binding domains and is seemingly unrelated to the enzymatic activity. In this study, we performed mass spectrometric analyses to elucidate the oligomeric states of AUH in the presence and absence of RNA. With a short RNA (AUUU) or without RNA, AUH… Expand
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