ATP is released from guinea pig ureter epithelium on distension.

@article{Knight2002ATPIR,
  title={ATP is released from guinea pig ureter epithelium on distension.},
  author={Gillian E. Knight and Philippe Bodin and William C. de Groat and Geoffrey Burnstock},
  journal={American journal of physiology. Renal physiology},
  year={2002},
  volume={282 2},
  pages={
          F281-8
        }
}
Distension of the perfused guinea pig ureter at pressures from 20 to 700 cmH(2)O increased the amount of ATP released from the epithelium in a pressure-dependent manner. During basal perfusion (40 microl/min), the perfusate contained 10 pmol/ml ATP; this increased 10- to 50-fold at various distending pressures. ATP was released from epithelial cells during distension as mechanical removal of the urothelium blocked release. No lactate dehydrogenase was detected in the perfusate, and scanning… 
View on PubMed
ucl.ac.uk
182 Citations
Highly Influential Citations
5
Background Citations
93
Methods Citations
1
Results Citations
5

Figures from this paper

182 Citations

Activation of ureter nociceptors by exogenous and endogenous ATP in guinea pig

ATP release from the human ureter on distension and P2X3 receptor expression on suburothelial sensory nerves

Immunofluorescence studies on consecutive frozen sections showed that suburothelial nerves stained positively for P2X3 and capsaicin receptors, with no staining in controls, consistent with the hypothesis that purinergic signalling is involved in human ureteric mechanosensory transduction, leading to nociception.

Kinetics of urothelial ATP release.

The bidirectional release of ATP by in vitro rabbit urothelium is investigated and the presence of a serosal ectonucleotidase/exon nucleotidases was demonstrated by the time-dependent decrease in exogenously added ATP.

ATP induces guinea pig gallbladder smooth muscle excitability via the P2Y4 receptor and COX-1 activity.

The data suggest that ATP stimulates P2Y(4) receptors within the gallbladder muscularis and, in turn, stimulate prostanoid production via COX-1 leading to increased excitability of GBSM.

Ion transport across Xenopus alveolar epithelium is regulated by extracellular ATP, UTP and adenosine

Hypotonic treatment evokes biphasic ATP release across the basolateral membrane of cultured renal epithelia (A6)

The data are consistent with the notion that during hypotonicity, basolateral ATP release activates purinergic receptors, which underlies the suramin‐sensitive rise of [Ca2+]i during the hyposmotic shock.

Hypotonicity-induced ATP release is potentiated by intracellular Ca2+ and cyclic AMP in cultured human bronchial cells.

Hypertonicity and changes in Cl(-) concentrations are not effective signals for the ATP release; hypotonicity-induced ATP release is potentiated by the level of intracellular Ca(2+) and cAMP; and a biphasic increase in ATP release and its low rate at the peak support the hypothesis that ATP is released through a non-conducting pathway model, such as exocytosis, or through a volume-dependent, ATP-conductive anion channel.

ATP and purinergic receptor-dependent membrane traffic in bladder umbrella cells.

Results indicate that increased hydrostatic pressure stimulates release of ATP from the uroepithelium and that upon binding to P2X and possibly P2Y receptors on the umbrella cell, downstream Ca2+ and PKA second messenger cascades may act to stimulate membrane insertion at the apical pole of these cells.

Purinergic component of mechanosensory transduction is increased in a rat model of colitis.

It was concluded that ATP has an enhanced role in mechanosensory transduction in the inflamed rat colorectum and the underlying mechanisms appear to involve increased distension-evoked release of ATP as well as an increase in the number of DRG neurons supplying the coloreCTum expressing P2X3 receptors, especially those containing calcitonin gene-related peptide.

Enhanced ATP release from rat bladder urothelium during chronic bladder inflammation: Effect of botulinum toxin A

...

References

SHOWING 1-10 OF 60 REFERENCES

ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes–possible sensory mechanism?

It is proposed that ATP is released from the urothelium as a sensory mediator for the degree of distension of the rabbit urinary bladder and other sensory modalities.

Increased flow‐induced ATP release from isolated vascular endothelial cells but not smooth muscle cells

The results show that, of the two major cell types of the vascular wall, only endothelial cells react to shear stress by releasing ATP, and there was no difference between lactate dehydrogenase activity in perfused cells and that in non‐perfused cells.

CFTR-independent ATP release from epithelial cells triggered by mechanical stimuli.

The rate of ATP release was unaffected by cAMP but was exquisitely sensitive to mechanical perturbations in both CFTR expressing and nonexpressing cells, suggesting Mechanically induced, CFTR-independent ATP release may be a physiologically relevant mechanism of epithelial regulation, which has not previously been fully appreciated.

Rapid release of endothelin and ATP from isolated aortic endothelial cells exposed to increased flow.

Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation.

Findings indicate that increases in cell volume lead to efflux of ATP through opening of a conductive pathway consistent with a channel, and that extracellular ATP is required for recovery from swelling.

Blockade by glibenclamide of the flow‐evoked endothelial release of ATP that contributes to vasodilatation in the pulmonary vascular bed of the rat

It is concluded that flow‐induced ATP release is mediated by a glibenclamide‐sensitive K+ channel, and that the release of ATP from endothelial cells probably functions to vasodilate the pulmonary vascular bed of the rat.

A release mechanism for stored ATP in ocular ciliary epithelial cells.

Both layers of the ciliary epithelium store and release ATP, and purines likely modulate aqueous humor flow by paracrine and/or autocrine mechanisms within the two cell layers of this epithelia.

An ATP‐sensitive potassium conductance in rabbit arterial endothelial cells.

The conductance of an ATP‐sensitive, sulphonylurea‐inhibitable potassium (K+) conductance in freshly dissociated endothelial cells from rabbit arteries was studied to establish an ionic selectivity sequence and the permeability was determined from reversal potential measurements.

ATP release from the isolated perfused guinea pig heart in response to increased flow.

It is concluded that ATP release from the guinea pig heart is increased under raised-flow conditions and it is suggested that this ATP release induces coronary vasodilatation.

Evidence for Gd3+ inhibition of membrane ATP permeability and purinergic signaling.

An important physiological role for constitutive release of ATP as a signal coordinating cell volume and membrane ion permeability is supported and it is suggested that Gd3+ might prove to be an effective inhibitor of ATP-permeable channels once they are identified.
...