ATP inhibits Mg(2+) uptake in MDCT cells via P2X purinoceptors.

  title={ATP inhibits Mg(2+) uptake in MDCT cells via P2X purinoceptors.},
  author={Lutao Dai and Hoon Kang and Dirk Kerstan and Gordon Ritchie and Gary A. Quamme},
  journal={American journal of physiology. Renal physiology},
  volume={281 5},
Nucleotides have diverse effects on water and electrolyte reabsorption within the distal tubule of the nephron. As the distal tubule is important in control of renal Mg(2+) balance, we determined the effects of ATP on cellular Mg(2+) uptake in this segment. The effects of ATP on immortalized mouse distal convoluted tubule (MDCT) cells were studied by measuring Mg(2+) uptake with fluorescence techniques. The mean basal Mg(2+) uptake rate was 165 +/- 6 nM/s. ATP inhibited basal Mg(2+) uptake and… CONTINUE READING