ATP-induced activation of expressed RyR3 at low free calcium.

  title={ATP-induced activation of expressed RyR3 at low free calcium.},
  author={Mario Manunta and Daniela Rossi and Ilenia Simeoni and Eugenio Butelli and Christoph Romanin and Vincenzo Sorrentino and Hansgeorg Schindler},
  journal={FEBS letters},
  volume={471 2-3},
Ca(2+) channel properties of the mink ryanodine receptor type 3 (RyR3), expressed in HEK293 cells, were studied in planar lipid bilayers to which RyR3 rich membrane fragments had been fused. RyR3 channels were not active at resting levels of Ca(2+)(free) but were gated by an additional 1 mM ATP, exhibiting long open times. The second major finding was the absence of channel inactivation at millimolar Ca(2+)(free). Insertion of a myc tag at the N-terminus of RyR3 did not affect the channel… CONTINUE READING

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