ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

@article{Ciechanover1980ATPdependentCO,
  title={ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.},
  author={Aaron Ciechanover and H B Heller and Sarah Elias and Amy Louise Haas and Avram Hershko},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 3},
  pages={1365-8}
}
The heat-stable polypeptide (APF-1) required for ATP-dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE-cellulose. Conjugate formation requires ATP and Mg2+ and its inhibited by N-ethylmaleimide. UTP and GTP are inactive. These properties are identical to those of ATP-dependent protein breakdown in the same system, suggesting that the conjugates are intermediates in this process. The APF… CONTINUE READING

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