ATP-dependent Ca2+ transport in vesicles isolated from the bile canalicular region of the hepatocyte plasma membrane.

@article{Bachs1985ATPdependentCT,
  title={ATP-dependent Ca2+ transport in vesicles isolated from the bile canalicular region of the hepatocyte plasma membrane.},
  author={Oriol Bachs and K S Famulski and Francesca Mirabelli and Ernesto Carafoli},
  journal={European journal of biochemistry},
  year={1985},
  volume={147 1},
  pages={1-7}
}
Three plasma membrane subfractions have been isolated and characterized from rat liver cells. The high affinity Ca2+-stimulated ATPase is highly enriched in the bile canalicular subfraction. Taking into account cross-contamination by the blood sinusoidal and lateral membranes it is suggested that the high-affinity Ca2+-ATPase is located exclusively in this fraction. The high-affinity Ca2+-ATPase is coupled to Ca2+ transport, is calmodulin-insensitive, sensitive to vanadate under appropriate… CONTINUE READING

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