ATP content of isolated fat cells. Effects of insulin, ouabain, and lipolytic agents.

@article{Bihler1970ATPCO,
  title={ATP content of isolated fat cells. Effects of insulin, ouabain, and lipolytic agents.},
  author={Ivan Bihler and Bernard Jeanrenaud},
  journal={Biochimica et biophysica acta},
  year={1970},
  volume={202 3},
  pages={
          496-506
        }
}
Abstract 1. 1. The ATP content of isolated fat cells was measured by a luminescence assay. 2. 2. The depression of ATP content induced by oligomycin was partly prevented by the addition of glucose to the incubation medium and more so by the further addition of insulin or ouabain, agents known to increase glucose uptake. 3. 3. Lipolytic agents (adrenaline, caffeine) markedly depressed the ATP levels. The magnitude of this effect was dependent on the concentrations used and on the duration of… 
Lipolysis and potassium accumulation in isolated fat cells. Effect of insulin and lipolytic agents.
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TLDR
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TLDR
It is suggested that a step in the coupling of beta-receptors and adenylate cyclase, but not total cellular cyclic AMP levels, may mediate stimulatory as well as inhibitory effects of catecholamines on glucose transport in the adipocyte.
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TLDR
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Metabolic effects of 2-deoxy-D-glucose in isolated fat cells.
TLDR
It is proposed that accumulation of 2-deoxyglucose 6-phosphate within fat cells has a direct inhibitory effect on cell respiration unrelated to inhibition of glycolysis.
Free fatty acid and ATP levels in adipocytes during lipolysis.
TLDR
The results strongly support the view that the fall in adipocyte ATP after treatment with lipolytic hormone is the result of intracellular FFA accumulation, which depresses ATP synthesis by uncoupling oxidative phosphorylation.
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Lipolysis and potassium accumulation in isolated fat cells. Effect of insulin and lipolytic agents.
TLDR
It is thought that insulin, in the absence of added glucose, prevents such a decrease in energy availability and/or production via its antilipolytic action, thereby counteracting the drop in 42K+ uptake induced by lipolytic hormones and agents.
Lipolysis and alpha-aminoisobutyric acid uptake in isolated fat cells. Effects of insulin and lipolytic agents.
TLDR
In the absence of glucose, insulin is thought to prevent, via its antilipolytic action, such a decrease in energy availability and/or production, thereby preventing the drop in α-aminoisobutyric acid uptake induced by lipolytic agents.
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