ATP and magnesium drive conformational changes of the Na+/K+-ATPase cytoplasmic headpiece.

  title={ATP and magnesium drive conformational changes of the Na+/K+-ATPase cytoplasmic headpiece.},
  author={Lenka Grycov{\'a} and Petr Sklenovsk{\'y} and Zdenek Lansky and Marika Janovsk{\'a} and Michal Otyepka and Ev{\vz}en Amler and Jan Teisinger and Martin Kubala},
  journal={Biochimica et biophysica acta},
  volume={1788 5},
Conformational changes of the Na(+)/K(+)-ATPase isolated large cytoplasmic segment connecting transmembrane helices M4 and M5 (C45) induced by the interaction with enzyme ligands (i.e. Mg(2+) and/or ATP) were investigated by means of the intrinsic tryptophan fluorescence measurement and molecular dynamic simulations. Our data revealed that this model system consisting of only two domains retained the ability to adopt open or closed conformation, i.e. behavior, which is expected from the crystal… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-9 of 9 extracted citations

Ion Pathways in the Na+/K+-ATPase

Journal of Chemical Information and Modeling • 2016

Na(+)/K(+)-ATPase inhibition by cisplatin and consequences for cisplatin nephrotoxicity.

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia • 2014


Publications referenced by this paper.
Showing 1-10 of 56 references


M. Hilge, G. Siegal, G. W. Vuister
Guntert, S.M. Gloor, J.P. Abrahams, ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPase, Nat. Struct. Biol. 10 • 2003
View 5 Excerpts
Highly Influenced


C. Toyoshima, M. Nakasako, H. Nomura
Ogawa, Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 angstrom resolution, Nature 405 • 2000
View 4 Excerpts
Highly Influenced

Further investigations on a Mg++ + Na+-activated adenosintriphosphatase

J. C. Skou
possibly related to the active, linked transport of Na+ and K+ across the nerve membrane, Biochim. Biophys. Acta 42 • 1960
View 3 Excerpts
Highly Influenced

Principles of Fluorescence Spectroscopy

R. Lakowicz
View 1 Excerpt

Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors.

Proceedings of the National Academy of Sciences of the United States of America • 2007
View 2 Excerpts

Similar Papers

Loading similar papers…