ATP and calcium modulation of nonselective cation channels in IMCD cells.

@article{Ono1994ATPAC,
  title={ATP and calcium modulation of nonselective cation channels in IMCD cells.},
  author={Seiitsu Ono and T Mougouris and Thomas D Dubose and Steven C. Sansom},
  journal={The American journal of physiology},
  year={1994},
  volume={267 4 Pt 2},
  pages={F558-65}
}
The mIMCD-3 cell line, developed from simian virus 40 transformed mice, was grown on coverslips for single-channel analysis of the apical membrane. An amiloride-sensitive nonselective cation channel (NCATP) was demonstrated that occurred predominantly in excised patches. The selectivity sequence for NCATP was NH4+ = Na+ = K+ = Li+ = Rb+ > Cl-. The single-channel conductance was 24 pS and nonrectifying in 140 mM KCl or NaCl solutions. NCATP was not permeable to barium from the extracellular side… CONTINUE READING