ATP and Energy Charge as Criteria of Growth and Metabolic Activity of Mollicutes : Application to Spiroplasma citri

@inproceedings{SAGLIO1978ATPAE,
  title={ATP and Energy Charge as Criteria of Growth and Metabolic Activity of Mollicutes : Application to Spiroplasma citri},
  author={By P. H. M. SAGLIO},
  year={1978}
}
  • By P. H. M. SAGLIO
  • Published 1978
Intracellular ATP concentrations and energy charge were monitored during the growth of Spiroplasma citri and related to the number of colony-forming units, to the pH of the medium and to incorporation of radioactive precursors. Of three different methods used for extracting nucleotides trichloroacetic acid, perchloric acid and boiling Tris/H,SO, buffer the trichloroacetic acid treatment was the most effective. During the active growth phase the ATP concentration increased exponentially, in the… CONTINUE READING
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