ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation.

@article{Stiff2004ATMAD,
  title={ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation.},
  author={Tom Stiff and Mark O'Driscoll and Nicole Rief and Kuniyoshi Iwabuchi and Markus L{\"o}brich and Penny A Jeggo},
  journal={Cancer research},
  year={2004},
  volume={64 7},
  pages={2390-6}
}
H2AX phosphorylation is an early step in the response to DNA damage. It is widely accepted that ATM (ataxia telangiectasia mutated protein) phosphorylates H2AX in response to DNA double-strand breaks (DSBs). Whether DNA-dependent protein kinase (DNA-PK) plays any role in this response is unclear. Here, we show that H2AX phosphorylation after exposure to ionizing radiation (IR) occurs to similar extents in human fibroblasts and in mouse embryo fibroblasts lacking either DNA-PK or ATM but is… CONTINUE READING