Corpus ID: 42736295

ATAstructure as having a causal role in the mechanism of TATATAA ; Roog 20 5 CCTTGTTTCT TTTAGGCGTTAGsilencing

  title={ATAstructure as having a causal role in the mechanism of TATATAA ; Roog 20 5 CCTTGTTTCT TTTAGGCGTTAGsilencing},
  author={R. Kamakaka and J. Rine},
A promoter fusion library of Saccharomyces cerevisiae genes was used to exploit phenotypes associated with altered protein dosage. We identified a novel gene, SAS10, by the ability of Sas10p, when overproduced, to disrupt silencing. The predicted Sas10p was 70,200 kD and strikingly rich in charged amino acids. Sas10p was exclusively nuclear in all stages of the cell cycle. Overproduction of Sas10p caused derepression of mating type genes at both HML and HMR, as well as of URA3, TRP1, and ADE2… Expand

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