ARF-TSS: an alternative method for identification of transcription start site in bacteria.

@article{Wang2012ARFTSSAA,
  title={ARF-TSS: an alternative method for identification of transcription start site in bacteria.},
  author={Chao Wang and Jasmine Yiqin Lee and Yinyue Deng and Fei Tao and Lian-Hui Zhang},
  journal={BioTechniques},
  year={2012},
  volume={52 4}
}
Current methods for identifying transcription start sites (TSSs) of specific genes in bacteria usually require adaptors or radioactive labeling. These approaches can be technically demanding and environmentally unfriendly. Here we present a method for identifying TSS called ARF-TSS, which is based on cDNA generation, circularization, PCR amplification, and DNA sequencing to determine the 5'-end of transcripts, thus circumventing the need for adaptors and radioactive labeling. We validated the… CONTINUE READING

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Current Protocols in Molecular Biology

  • F. M. Ausubel, R. Brent, +4 authors K. Struhl
  • 1994

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