AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

  title={AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach},
  author={Hannes M. Beyer and Patrick Gonschorek and Sophia L. Samodelov and Matthias Meier and Wilfried Weber and Matias D. Zurbriggen and Dipankar Chatterji},
  booktitle={PloS one},
Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we… CONTINUE READING
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