• Corpus ID: 4826046

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ALOGLIPTIN AND METFORMIN HYDROCHLORIDE TABLET DOSAGE FORM BY RP-HPLC METHOD

@inproceedings{Kumar2013ANALYTICALMD,
  title={ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ALOGLIPTIN AND METFORMIN HYDROCHLORIDE TABLET DOSAGE FORM BY RP-HPLC METHOD},
  author={A. Praveen Kumar and Gorusupudi Aruna and K. Rajasekar and P. Jaya Chandra Reddy},
  year={2013}
}

• A. Kumar, G. Aruna, +1 author P. Reddy
• Published 2013
• Chemistry

In the present work, RP-HPLC method for two drugs have been developed and validated for simultaneous determination of Alogliptin and Metformin Hydrochloride in Tablet dosage form. In this method, 0.2% TEA pH adjusted with OPA to 6.0 was used as buffer mobile phase was prepared by adjusting the ratio of buffer and Methanol as 30:70 v/v + 0.2% Triethylamine and a flow rate of 1.0 ml/min. The optimum wavelength for detection was 254 nm and a run time of 10 minutes was used. The column used was Agilent C18 with dimension 250 mm length, 4.6 mm i.d., 5µ particle size. The method was validated for its linearity, accuracy, precision, specificity, robustness. The system suitability parameters passed and linearity was observed in the range of 25-150 µg/ml for Alogliptin and Metformin Hydrochloride respectively and the correlation coefficient of Alogliptin and Metformin Hydrochloride were 0.9995 and 0.9996. The accuracy was performed and the % recovery was observed as 99.96% and 99.83% for Alogliptin and Metformin Hydrochloride respectively. Thus a novel, sensitive, accurate, specific, precise method was developed for the Simultaneous determination of Alogliptin and Metformin Hydrochloride in tablet dosage form by RP-HPLC Method.