• Corpus ID: 37618955

AMPA, NMDA and GABAA receptor mediated network burst dynamics in cortical cultures in vitro

  title={AMPA, NMDA and GABAA receptor mediated network burst dynamics in cortical cultures in vitro},
  author={Heidi Teppola and Samora Okujeni and Marja-Leena Linne and U. Egert Department of Signal Processing and Tampere University of Technology and Tampere and Finland. and Bernstein Center FreiburgDepartment of Microsystems Engineering and Imtek and Albert-Ludwig University of Freiburg and Freiburg and Germany},
  journal={arXiv: Neurons and Cognition},
In this work we study the excitatory AMPA, and NMDA, and inhibitory GABAA receptor mediated dynamical changes in neuronal networks of neonatal rat cortex in vitro. Extracellular network-wide activity was recorded with 59 planar electrodes simultaneously under different pharmacological conditions. We analyzed the changes of overall network activity and network-wide burst frequency between baseline and AMPA receptor (AMPA-R) or NMDA receptor (NMDA-R) driven activity, as well as between the latter… 

Figures from this paper

Unique Features of Network Bursts Emerge From the Complex Interplay of Excitatory and Inhibitory Receptors in Rat Neocortical Networks

This study is the first to unravel in detail how the three main mediators of synaptic transmission uniquely shape the NB characteristics, such as the initiation, maintenance, recruitment and termination of NBs in cortical cell cultures in vitro.

Patient-Derived Anti-NMDAR Antibody Disinhibits Cortical Neuronal Networks through Dysfunction of Inhibitory Neuron Output

It is revealed that autoantibody against the NMDA receptor (NMDAR) has a distinct mechanism of action in the cortex, where it impairs function of inhibitory neurons leading to increased cortical network excitability, in contrast to previously described hippocampal synaptic mechanisms of information encoding, highlighting brain regional specificity.

27th Annual Computational Neuroscience Meeting (CNS*2018): Part One

The K1 Probabilistic models of sensorimotor control and decision making help clarify the role of emotion in decision making and provide a basis for future generations of decision-making models to be based on.

Reproducibility and Rigour in Computational Neuroscience

The construction of a pipeline centered around an intermediate representation, termed dLEMS, which would facilitate simplified code generation not only for neuronal simulators but also for ODE-aware general purpose numerical platforms like Matlab or even C/Sundials, and the interplay between syntax and semantics is illustrated.

Challenges in Reproducibility, Replicability, and Comparability of Computational Models and Tools for Neuronal and Glial Networks, Cells, and Subcellular Structures

The comparability of a versatile choice of simulation tools: tools for biochemical reactions and spiking neuronal networks, and relatively new tools for growth in cell cultures, and the need for developing recommendations and good practices for publishing simulation tools and computational models are highlighted.



Contributions of NMDA receptors to network recruitment and rhythm generation in spinal cord cultures

It is concluded that NMDA‐R contribute to rhythm generation in spinal cultures by supporting recurrent excitation and network recruitment and by depolarizing the network.

The dynamics of a neuronal culture of dissociated cortical neurons of neonatal rats

The results presented here show that the neuronal network may exist in two different dynamical states: one state in which the neuron network behaves as a non-chaotic deterministic system and another state where the system exhibits large spatio-temporal fluctuations, characteristic of stochastic or chaotic systems.

Periodic synchronized bursting and intracellular calcium transients elicited by low magnesium in cultured cortical neurons.

In Mg(2+)-free external solution, rat cortical neurons in cultured networks entered a stable firing mode, consisting of regular bursts of action potentials superimposed on long-lasting depolarizations, showing that periodic synaptic input is responsible for the burst-depolarizations.

Dynamics and Effective Topology Underlying Synchronization in Networks of Cortical Neurons

The activity of early-to-fire neurons reliably forecasts an upcoming network spike and provides means for expedited propagation between assemblies, and theory predicts that scale-free topology allows for synchronization time that does not increase markedly with network size; this prediction is supported.

An extremely rich repertoire of bursting patterns during the development of cortical cultures

Dissociated cultures of cortical cells exhibited a much richer repertoire of activity patterns than previously reported, except for the very sparsest cultures, which exhibited globally synchronized bursts, but bursting patterns changed over the course of development, and varied considerably between preparations.

Biological application of microelectrode arrays in drug discovery and basic research

Examples of MEA biosensor applications are described that have been developed for drug screening and discovery and safety pharmacology in the field of cardiac and neural research and biophysical basics of recording and concepts for analysis of extracellular electrical signals are presented.

and s

Impulse activity and the patterning of connections during cns development