AMP-activated protein kinase undergoes nucleotide-dependent conformational changes

  title={AMP-activated protein kinase undergoes nucleotide-dependent conformational changes},
  author={L. Chen and J. Wang and Y. Zhang and S. F. Yan and D. Neumann and U. Schlattner and Z. Wang and J. Wu},
  journal={Nature Structural &Molecular Biology},
  • L. Chen, J. Wang, +5 authors J. Wu
  • Published 2012
  • Chemistry, Medicine
  • Nature Structural &Molecular Biology
  • The energy sensor AMP-activated protein kinase (AMPK) is a heterotrimeric complex that is allosterically activated by AMP binding to the γ subunit. Cocrystal structures of the mammalian AMPK core reveal occlusion of nucleotide-binding site 3 of the γ subunit in the presence of ATP. However, site 3 is occupied in the presence of AMP. Mutagenesis studies indicate that sites 3 and 4 are important for AMPK allosteric activation. 
    99 Citations

    Figures, Tables, and Topics from this paper

    Explore Further: Topics Discussed in This Paper

    Molecular Mechanism of Nucleotide-Dependent Allosteric Regulation in AMP-Activated Protein Kinase.
    • 1
    ATP sensitive bi-quinoline activator of the AMP-activated protein kinase.
    • 6
    Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK.
    • 3
    Activation of AMP-activated protein kinase revealed by hydrogen/deuterium exchange mass spectrometry.
    • 33
    • Highly Influenced
    Structural basis of AMPK regulation by adenine nucleotides and glycogen
    • 118
    • PDF
    Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization.
    • 2
    Bypassing AMPK phosphorylation.
    • 12
    • PDF


    AMP-activated protein kinase: also regulated by ADP?
    • 151
    Structural basis for AMP binding to mammalian AMP-activated protein kinase
    • 484
    AMPK Is a Direct Adenylate Charge-Regulated Protein Kinase
    • 466
    β-Subunit myristoylation is the gatekeeper for initiating metabolic stress sensing by AMP-activated protein kinase (AMPK)
    • 259
    • PDF
    Structure of Mammalian AMPK and its regulation by ADP
    • 698
    • PDF