AMP-activated protein kinase undergoes nucleotide-dependent conformational changes
@article{Chen2012AMPactivatedPK, title={AMP-activated protein kinase undergoes nucleotide-dependent conformational changes}, author={L. Chen and J. Wang and Y. Zhang and S. F. Yan and D. Neumann and U. Schlattner and Z. Wang and J. Wu}, journal={Nature Structural &Molecular Biology}, year={2012}, volume={19}, pages={716-718} }
The energy sensor AMP-activated protein kinase (AMPK) is a heterotrimeric complex that is allosterically activated by AMP binding to the γ subunit. Cocrystal structures of the mammalian AMPK core reveal occlusion of nucleotide-binding site 3 of the γ subunit in the presence of ATP. However, site 3 is occupied in the presence of AMP. Mutagenesis studies indicate that sites 3 and 4 are important for AMPK allosteric activation.
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- 13
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References
SHOWING 1-10 OF 21 REFERENCES
Crystal Structures of the Adenylate Sensor from Fission Yeast AMP-Activated Protein Kinase
- Biology, Medicine
- Science
- 2007
- 177
- PDF
AMP-activated protein kinase: also regulated by ADP?
- Biology, Medicine
- Trends in biochemical sciences
- 2011
- 151
Structural basis for AMP binding to mammalian AMP-activated protein kinase
- Biology, Medicine
- Nature
- 2007
- 484
β-Subunit myristoylation is the gatekeeper for initiating metabolic stress sensing by AMP-activated protein kinase (AMPK)
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences
- 2010
- 259
- PDF
Structural insight into the autoinhibition mechanism of AMP-activated protein kinase
- Biology, Medicine
- Nature
- 2009
- 184
Dissecting the Role of 5′-AMP for Allosteric Stimulation, Activation, and Deactivation of AMP-activated Protein Kinase*
- Chemistry, Medicine
- Journal of Biological Chemistry
- 2006
- 441
- PDF
Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.
- Biology, Medicine
- Structure
- 2011
- 29