AM251 induces apoptosis and G2/M cell cycle arrest in A375 human melanoma cells

  title={AM251 induces apoptosis and G2/M cell cycle arrest in A375 human melanoma cells},
  author={Sara Carpi and Stefano Fogli and Antonella Romanini and Mario Pellegrino and Barbara Adinolfi and Adriano Podest{\`a} and Barbara Costa and Eleonora Da Pozzo and Claudia Martini and Maria Cristina Breschi and Paola Nieri},
  journal={Anti-Cancer Drugs},
Human cutaneous melanoma is an aggressive and chemotherapy-resistant type of cancer. AM251 is a cannabinoid type 1 (CB1) receptor antagonist/inverse agonist with off-target antitumor activity against pancreatic and colon cancer cells. The current study aimed to characterize the in-vitro antimelanoma activity of AM251. The BRAF V600E mutant melanoma cell line, A375, was used as an in-vitro model system. Characterization tools included a cell viability assay, nuclear morphology assessment, gene… 
Tumor-promoting effects of cannabinoid receptor type 1 in human melanoma cells.
  • S. Carpi, S. Fogli, P. Nieri
  • Biology
    Toxicology in vitro : an international journal published in association with BIBRA
  • 2017
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Sinularin induces oxidative stress‐mediated G2/M arrest and apoptosis in oral cancer cells
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Anticancer Activity of Euplotin C, Isolated from the Marine Ciliate Euplotes crassus, Against Human Melanoma Cells
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Sulfonyl chromen-4-ones (CHW09) shows an additive effect to inhibit cell growth of X-ray irradiated oral cancer cells, involving apoptosis and ROS generation
CHW09/X-ray treatment had additive growth inhibiting effects against X-ray irradiated oral cancer cells, partly attributing to apoptosis and ROS generation.
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Cannabinoids as Anticancer Drugs.


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The molecular beacon-oligodeoxynucleotide tested in the current study conjugates the ability of imaging with the pharmacological silencing activity against survivin mRNA in human melanoma cells and may represent an innovative approach for cancer diagnosis and treatment.
Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth Inhibition, G2-M arrest, and apoptosis.
Findings suggest a need for in vivo studies with this combination of silibinin and doxorubicin against prostate cancer, which is limited because of high systemic toxicity, and might be relevant for a clinical application in prostate cancer patients.
Benzyl isothiocyanate (BITC) induces G2/M phase arrest and apoptosis in human melanoma A375.S2 cells through reactive oxygen species (ROS) and both mitochondria-dependent and death receptor-mediated multiple signaling pathways.
The results showed that BITC promoted the induction of G(2)/M phase arrest and apoptosis in A375.S2 human melanoma cells through ER stress- and mitochondria-dependent and death receptor-mediated multiple signaling pathways, and suggest thatBITC has potential as an agent for the treatment of melanoma.
Revisiting CB1 Receptor as Drug Target in Human Melanoma
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TRPA1 is functionally expressed in melanoma cells but is not critical for impaired proliferation caused by allyl isothiocyanate or cinnamaldehyde
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Cannabinoid receptors as novel targets for the treatment of melanoma
Evaluated the efficacy of cannabinoid receptor agonists, a new family of potential antitumoral compounds, at skin melanoma, and found that Cannabinoid antiproliferative action on melanoma cells was due, at least in part, to cell cycle arrest at the G1‐S transition.