ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens.

@article{Huth2005ALARMNA,
  title={ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens.},
  author={Jeffrey R. Huth and Renaldo Mendoza and Edward T. Olejniczak and Robert W. Johnson and Darlene A Cothron and Yaya Liu and Claude G Lerner and Jun L. Chen and Philip J. Hajduk},
  journal={Journal of the American Chemical Society},
  year={2005},
  volume={127 1},
  pages={217-24}
}
High-throughput screening (HTS) of large compound collections typically results in numerous small molecule hits that must be carefully evaluated to identify valid drug leads. Although several filtering mechanisms and other tools exist that can assist the chemist in this process, it is often the case that costly synthetic resources are expended in pursuing false positives. We report here a rapid and reliable NMR-based method for identifying reactive false positives including those that oxidize… CONTINUE READING