AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditsribution

  title={AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditsribution},
  author={P. Castiglioni and Paolo Ajmone-Marsan and Ringo van Wijk and Mario Motto},
  journal={Theoretical and Applied Genetics},
We exploited the AFLP technique to saturate a RFLP linkage map derived from a maize mapping population. By using two restriction enzyme, EcoRI and PstI, differing in methylation sensitivity, both in combination with MseI, we detected 1568 bands of which 340 where polymorphic. These were added to the exitsing RFLP marker data to study the effects of incorporation of AFLPs produced by different restriction-enzyme combinations upon genetic maps. Addition of the AFLP data resulted in greater genome… CONTINUE READING
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