AFFINITY BIOSEPARATION AND BIOSENSING USING MAGNETIC PARTICLES FOR FOOD SAFETY PhD Thesis

Abstract

A very simple and rapid method for the detection of Salmonella in milk is reported. In this approach,<lb>the bacteria are captured and preconcentrated from milk samples with magnetic beads through an<lb>immunological reaction. A second polyclonal antibody labeled with peroxidase is used as serological<lb>confirmation with electrochemical detection based on a magneto-electrode. The ‘IMS/m-GEC electro-<lb>chemical immunosensing’ approach shows a limit of detection of 5×103 and 7.5×103 CFUmL−1 in LB eywords:<lb>lectrochemical immunosensor<lb>mmunomagnetic separation<lb>almonella<lb>and in milk diluted 1/10 in LB broth, respectively, in 50min without any pretreatment. If the skimmed-<lb>milk is preenriched for 6h, the method is able to detect as low as<lb>1.4CFUmL−1, while if it is preenriched<lb>for 8h, as low as 0.108×CFUmL−1 (2.7×CFU in 25g of milk, in 5 samples of 5mL) are detected accord-<lb>ingly with the legislation. Moreover, the method is able to clearly distinguish between food pathogenic<lb>la and<lb>thods<lb>scherichia coli<lb>agnetic bead<lb>agneto-electrode<lb>bacteria such as Salmonel<lb>with classical culture me

Cite this paper

@inproceedings{GIRONA2013AFFINITYBA, title={AFFINITY BIOSEPARATION AND BIOSENSING USING MAGNETIC PARTICLES FOR FOOD SAFETY PhD Thesis}, author={SUSANA LI{\'E}BANA GIRONA and Mar{\'i}a Isabel Pividori and Salvador Alegret and Susana Li{\'e}bana Girona and Susana Li{\'e}bana and Denis Augusto Spricigo and Joan Colom and Susana Campoy and P Vidal Cort{\'e}s and Montserrat Llagostera and Jordi Barb{\'e} and O. Castell and Silvia R. Hern{\'a}ndez and Mart{\'i}n Jos{\'e} Zum{\'a}rraga and Menno W J Prins}, year={2013} }