ADP-ribosylation of proteins in non-infected Escherichia coli cells.

Partially purified enzymatic fractions from extracts of Escherichia coli B/r catalyse transfer of the isotope label from [adenine-2,8-(3)H]NAD+ to some bacterial proteins, as well as to hen egg-white lysozyme. The radioactive group in the modified lysozyme was identified as mono(ADP-ribose). Several bacterial proteins were labelled in vivo with 32P; the… CONTINUE READING