ADP-ribosylation of adenylate cyclase by pertussis toxin. Effects on inhibitory agonist binding.

@article{Hsia1984ADPribosylationOA,
  title={ADP-ribosylation of adenylate cyclase by pertussis toxin. Effects on inhibitory agonist binding.},
  author={Judith A Hsia and Joel Moss and Erik L. Hewlett and Martha Vaughan},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 2},
  pages={1086-90}
}
Adenylate cyclase in NG108-15 (neuroblastoma X glioma hybrid) cells is responsive to both stimulatory and inhibitory ligands. Bordetella pertussis toxin (PT) catalyzes the ADP-ribosylation of a 41,000-Da peptide believed to be a subunit of the putative guanyl nucleotide-binding protein (Gi) involved in cyclase inhibition and abolishes inhibitory effects of opiate agonists. In studying the effects of PT on opiate receptors, we found that [3H]enkephalinamide binding was reduced by approximately… CONTINUE READING

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