ADP release from myosin in permeabilized smooth muscle.

  title={ADP release from myosin in permeabilized smooth muscle.},
  author={Thomas M. Butler and Denise S Pac{\'i}fico and Marion J. Siegman},
  journal={The American journal of physiology},
  volume={256 1 Pt 1},
The purpose of this study was to determine the nucleotide bound to myosin and its rate of release under relaxed and activated conditions in permeabilized rabbit portal veins. Incubation of the muscles in a relaxing solution containing [3H]-ATP resulted in the formation of 60-70 microM radiolabeled ADP in the muscle whether or not the myosin light chains had been thiophosphorylated. This value was similar to the estimate of the concentration of myosin subfragment 1. Upon transfer of the muscles… CONTINUE READING