ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy

@article{Sosa2001ADPinducedRO,
  title={ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy},
  author={Hernando J. Sosa and Erwin J. G. Peterman and W E Moerner and Lawrence S. B. Goldstein},
  journal={Nature Structural Biology},
  year={2001},
  volume={8},
  pages={540-544}
}
Kinesin is an ATP-driven molecular motor protein that moves processively along microtubules. Despite considerable research, the detailed mechanism of kinesin motion remains elusive. We applied an enhanced suite of single- and multiple-molecule fluorescence polarization microscopy assays to report the orientation and mobility of kinesin molecules bound to microtubules as a function of nucleotide state. In the presence of analogs of ATP, ADP-Pi or in the absence of nucleotide, the kinesin head… CONTINUE READING

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