ACTION OF X-RAYS ON MAMMALIAN CELLS

@article{Puck1956ACTIONOX,
  title={ACTION OF X-RAYS ON MAMMALIAN CELLS},
  author={T. Puck and P. I. Marcus},
  journal={The Journal of Experimental Medicine},
  year={1956},
  volume={103},
  pages={653 - 666}
}
The effects of x-irradiation have been quantitatively studied on single cells of a human cervical carcinoma (HeLa) under conditions such that 100 per cent of the unirradiated cells reproduce in isolation to form macroscopic colonies. This technique eliminates complexities due to interactions of members of large cell populations. Survival of single cells (defined as the ability to form a macroscopic colony within 15 days) yields a typical 2 hit curve when plotted against x-ray dose. The initial… Expand
Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.
TLDR
A study was undertaken, using two-cell lines of the Chinese hamster propagated in vitro, to determine whether or not sublethal damage is heritsble, as might be expected if lethal damage is primarily genic. Expand
X-ray-induced chromosome damage in live mammalian cells, and improved measurements of its effects on their colony-forming ability.
TLDR
Improved the precision of the technique described by Grote et al. (1981 a,b) for the observation of the radiation responses of live cultured mammalian cells with an incubated phase-contrast microscope and the colony-forming abilities of single cells obtained by selective detachment of mitoses may be followed individually and may be directly compared with chromosome damage detected after post-radiation mitosis. Expand
RADIOMIMETIC EFFECTS OF A NITROGEN MUSTARD ON SURVIVAL , GROWTH, PROTEIN AND NUCLEIC ACID SYNTHESIS OF MAMMALIAN CELLS IN VITRO.
Abstract The effects of different doses of a nitrogen mustard (HN2) on a guinea-pig cell strain grown in vitro , have been compared to the effects of X-rays on similar systems. 1. 1. The survivalExpand
MODIFICATION OF RADIATION DAMAGE BY FRACTIONATION OF THE DOSE, ANOXIA, AND CHEMICAL PROTECTORS IN RELATION TO LET
  • G. Barendsen
  • Chemistry, Medicine
  • Annals of the New York Academy of Sciences
  • 1964
TLDR
Studies of effects produced by ionizing radiations of different linear energy transfer (LET) on the proliferative capacity of cells are of interest for several reasons, including the use of ionizing radiation in the treatment of cancer. Expand
DOSE RATE: ITS EFFECT ON THE SURVIVAL OF HELA CELLS IRRADIATED WITH GAMMA RAYS.
TLDR
The aim of the investigation was to determine whether the dose-rate effect commonly observed with small mammals could be fully accounted for at the cellular level, or whether a systemic effect on the whole animal was involved. Expand
A Multicomponent X-ray Survival Curve for Mouse Lymphosarcoma Cells irradiated in vivo
NEARLY all investigations hitherto reported of the survival of animal cell populations exposed to single doses of ionizing radiations have yielded data which have been fitted by curves of the sameExpand
RESPONSE OF NEOPLASTIC CELLS IN VITRO TO IONIZING RADIATION *
  • A. Goldfeder
  • Biology, Medicine
  • Annals of the New York Academy of Sciences
  • 1961
TLDR
Evidence for a selective response to irradiation between various types of tissue cells, normal and malignant, is presented and studies on a subcellular level revealed that the mitochondria of the more radiosensitive tumor are fewer in number and inferior in quality as compared to that of theMore radioresistant tumor. Expand
The action of caffeine on X-irradiated HeLa cells. VII. Evidence that caffeine enhances expression of potentially lethal radiation damage.
TLDR
It is concluded that X rays do not sensitize cells to caffeine, but rather that caffeine enhances the expression of potentially lethal radiation-induced damage. Expand
ACTION OF RADIATION ON MAMMALIAN CELLS III. RELATIONSHIP BETWEEN REPRODUCTIVE DEATH AND INDUCTION OF CHROMOSOME ANOMALIES BY X-IRRADIATION OF EUPLOID HUMAN CELLS IN VITRO.
  • T. Puck
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1958
TLDR
Data support the previously advanced interpretation that the primary process leading to destruction of the reproductive ability of cultured single mammalian cells by x irradiation is a damage to the chromosomes, and place an upper limit of 40 to 60 r on the value of the dose needed to produce an average of one chromosome abnormality in an euploid, fibroblast-like human cell under these conditions. Expand
X-irradiation response by TD-50 and survival time assays for a murine lymphoma.
TLDR
In these experiments, a lymphomatous tumor cell growth model, previously described, has been used for purposes of interpretation and it is known that a certain level of fractional survival can be expected. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 23 REFERENCES
ACTION OF X-RAYS ON MAMMALIAN CELLS
TLDR
Survival curves of normal human cells from a variety of tissues exposed to varying doses of x-irradiation have been constructed, which permit definition of the intrinsic radiation sensitivity of the reproductive power of each cell type, and afford a convincing explanation of several aspects of the mammalian radiation syndrome. Expand
Actions of Radiations on Living Cells
THIS book is the most important and authoritative work now available dealing with the simplest and most indepantal actions of ionizing radiations on living cells. The scope is intentionally limited.Expand
Topley and Wilson's Principles of Bacteriology and Immunity
THIS edition, the third in seventeen years, following four printings of the first and seven of the second edition, indicates a large and steady demand for the work. The late Prof. Topley hadExpand
J. Exp. Med
  • J. Exp. Med
  • 1956
Cancer J. B., Cancer
  • Cancer J. B., Cancer
  • 1955
Proc. Nat. Acad. Sc
  • Proc. Nat. Acad. Sc
  • 1955
Animal Virology, New York
  • 1953
J. Exp. Meal
  • J. Exp. Meal
  • 1953
Bact
  • Bact
  • 1952
...
1
2
3
...