AC133+ umbilical cord blood progenitors demonstrate rapid self-renewal and low apoptosis.

@article{Forraz2002AC133UC,
  title={AC133+ umbilical cord blood progenitors demonstrate rapid self-renewal and low apoptosis.},
  author={Nicolas Forraz and Ruth Pettengell and Pierre-Antoine Deglesne and Colin P. McGuckin},
  journal={British journal of haematology},
  year={2002},
  volume={119 2},
  pages={516-24}
}
Umbilical cord blood (UCB) provides immediate access to haemopoietic stem/progenitor cells (HSPC) but low cell number restricts use in full adult bone marrow reconstitution. This study investigated early ex vivo expansion kinetics of UCB AC133+ cells (2-4 x 10(4)/ml), mononuclear cells (MNC, 1-2 x 10(6)/ml) and AC133negative cells (AC133(neg), 2-4 x 10(4)/ml) in stroma-free 8 d liquid culture (fetal bovine serum-supplemented Iscove's-modified Dulbecco's medium (IMDM) with either 'K36EG'[c-Kit… CONTINUE READING