AAVS1-Targeted Plasmid Integration in AAV Producer Cell Lines.

Abstract

Adeno-associated virus (AAV) producer cell lines are created via transfection of HeLaS3 cells with a single plasmid containing three components (the vector sequence, the AAV rep and cap genes, and a selectable marker gene). As this plasmid contains both the cis (Rep binding sites) and trans (Rep protein encoded by the rep gene) elements required for site… (More)
DOI: 10.1089/hgtb.2016.158

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