AAV-mediated gene targeting methods for human cells

  title={AAV-mediated gene targeting methods for human cells},
  author={Iram F. Khan and Roli K. Hirata and David W. Russell},
  journal={Nature Protocols},
Gene targeting with adeno-associated virus (AAV) vectors has been demonstrated in multiple human cell types, with targeting frequencies ranging from 10−5 to 10−2 per infected cell. These targeting frequencies are 1–4 logs higher than those obtained by conventional transfection or electroporation approaches. A wide variety of different types of mutations can be introduced into chromosomal loci with high fidelity and without genotoxicity. Here we provide a detailed protocol for gene targeting in… CONTINUE READING
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