AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition.

@article{Erdmann2011AAAAP,
  title={AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition.},
  author={Susanne Erdmann and Urte Scheele and Roger A. Garrett},
  journal={Virology},
  year={2011},
  volume={421 1},
  pages={61-6}
}
The two structural domains of p529, a predicted AAA ATPase of Acidianus two-tailed virus (ATV), were expressed and purified. The N-terminal domain was demonstrated by loss-of-function mutations to carry ATPase activity with a temperature optimum of 60°C. This domain also showed DNA binding activity that was stronger for the whole protein and was weakened in the presence of ATP. The C-terminal domain exhibits Mg(2+)-dependent endonuclease activity that was eliminated by site-directed mutagenesis… CONTINUE READING

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