A82w Improves Regioselectivity of Steroid Hydroxylation by Cytochrome P450 Bm3 Mutants as Rationalized by Spin Relaxation Nmr Studies

Abstract

Cytochrome P450 BM3 from Bacillus megaterium is a monoxygenase with great potential for biotechnological applications. In this work, we present engineered drug metabolizing P450 BM3 mutants as a novel tool for regioselective hydroxylation of steroids at position 16ß. In particular, we show that by replacing alanine at position 82 with a tryptophan in P450… (More)

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