A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway

@article{Shi2003A3AA,
  title={A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a G$\beta$$\gamma$‐coupled MAPK signaling pathway},
  author={Chanjuan Shi and A M Szczesniak and Lucy Mao and Christine A. B. Jollimore and Miguel Coca-Prados and Orlando Hung and Melanie E. M. Kelly},
  journal={British Journal of Pharmacology},
  year={2003},
  volume={139}
}
We examined A3 adenosine and CB1 cannabinoid receptor‐coupled signaling pathways regulating Cl− current in a human nonpigmented ciliary epithelial (NPCE) cell line. Whole‐cell patch‐clamp recordings demonstrated that the A3 receptor agonist, IB‐MECA, activates an outwardly rectifying Cl−current (ICl,Aden) in NPCE cells, which was inhibited by the adenosine receptor antagonist, CGS‐15943 or by the protein kinase C (PKC) activator, phorbol 12,13 dibutyrate (PDBu). Treatment of NPCE cells with… Expand
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