A1 adenosine receptors. Two amino acids are responsible for species differences in ligand recognition.

@article{Tucker1994A1AR,
  title={A1 adenosine receptors. Two amino acids are responsible for species differences in ligand recognition.},
  author={Amy L. Tucker and A S Robeva and Harry E. Taylor and D Holeton and M Bockner and Kevin R Lynch and Joel M Linden},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 45},
  pages={27900-6}
}
Species differences in ligand binding to A1 adenosine receptors were localized to the seventh transmembrane (TM7) region based on the binding of [8-3H]cyclopentyl-1, 3-dipropylxanthine and three other ligands to wild type and six bovine/canine interspecies receptor chimeras expressed in COS-1 cells. Subsequent site-directed mutagenesis experiments identified amino acid 270 (isoleucine/methionine, bovine/canine) as being primarily responsible for species differences in the binding of N6-adenine… CONTINUE READING