A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications

@inproceedings{Kim2011AZF,
  title={A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications},
  author={Moon-Soo Kim and Gulnaz Stybayeva and Ji Youn Lee and Alexander Revzin and David J Segal},
  booktitle={Nucleic acids research},
  year={2011}
}
The visual detection of specific double-stranded DNA sequences possesses great potential for the development of diagnostics. Zinc finger domains provide a powerful scaffold for creating custom DNA-binding proteins that recognize specific DNA sequences. We previously demonstrated sequence-enabled reassembly of TEM-1 β-lactamase (SEER-LAC), a system consisting of two inactive fragments of β-lactamase each linked to engineered zinc finger proteins (ZFPs). Here the SEER-LAC system was applied to… CONTINUE READING