A zinc finger nuclease assay to rapidly quantitate homologous recombination proficiency in human cell lines.

@article{Yuan2013AZF,
  title={A zinc finger nuclease assay to rapidly quantitate homologous recombination proficiency in human cell lines.},
  author={Jing Yuan and Peter R. Strack and Carlo Toniatti and Marc Pelletier},
  journal={Analytical biochemistry},
  year={2013},
  volume={434 1},
  pages={96-8}
}
Homologous recombination (HR) is a cellular mechanism for accurate repair of double-strand DNA breaks, often deregulated in cancer. Development of novel cancer therapeutics targeting HR pathways would benefit from a quantitative and rapid means of measuring HR. Here, we describe a zinc finger nuclease (ZFN) assay that can quantify HR. Knockdown of BRCA1 or inactivation of BRCA2 decreased HR activity in cells, whereas gene restoration induced activity 8-fold. HR activity was also reflective of… CONTINUE READING
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