A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABAA receptor chloride channels.

@article{Kruger2005AYF,
  title={A yellow fluorescent protein-based assay for high-throughput screening of glycine and GABAA receptor chloride channels.},
  author={Wade A Kruger and Daniel J Gilbert and Rebecca L Hawthorne and Deanne H. Hryciw and Stephan Frings and Philip Poronnik and Joseph W Lynch},
  journal={Neuroscience letters},
  year={2005},
  volume={380 3},
  pages={340-5}
}
There is a significant clinical need to identify novel ligands with high selectivity and potency for GABA(A), GABA(C) and glycine receptor Cl- channels. Two recently developed, yellow fluorescent protein variants (YFP-I152L and YFP-V163S) are highly sensitive to quench by small anions and are thus suited to reporting anionic influx into cells. The aim of this study was to establish the optimal conditions for using these constructs for high-throughput screening of GABA(A), GABA(C) and glycine… CONTINUE READING
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