A yeast sensor of ligand binding

  title={A yeast sensor of ligand binding},
  author={Chandra L. Tucker and Stanley Fields},
  journal={Nature Biotechnology},
We describe a biosensor that reports the binding of small-molecule ligands to proteins as changes in growth of temperature-sensitive yeast. The yeast strains lack dihydrofolate reductase (DHFR) and are complemented by mouse DHFR containing a ligand-binding domain inserted in a flexible loop. Yeast strains expressing two ligand-binding domain fusions, FKBP12-DHFR and estrogen receptor-α (ERα)-DHFR, show increased growth in the presence of their corresponding ligands. We used this sensor to… CONTINUE READING
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