A workingperson's guide to deconvolution in light microscopy.

  title={A workingperson's guide to deconvolution in light microscopy.},
  author={William Wallace and L. J. Schaefer and Jason R. Swedlow},
  volume={31 5},
  pages={1076-8, 1080, 1082 passim}
Thefluorescence microscope is routinely used to study cellular structure in many biomedical research laboratories and is increasingly used as a quantitative assay system for cellular dynamics. One of the major causes of image degradation in the fluorescence microscope is blurring. Deconvolution algorithms use a model of the microscope imaging process to either subtract or reassign out-of-focus blur. A variety of algorithms are now commercially available, each with its own characteristic… CONTINUE READING
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