A wide range of protein isoforms in serum and plasma uncovered by a quantitative intact protein analysis system.

@article{Misek2005AWR,
  title={A wide range of protein isoforms in serum and plasma uncovered by a quantitative intact protein analysis system.},
  author={David E. Misek and Rork Kuick and Hong Wang and Vladimir Galchev and Bin Deng and Rong Zhao and John Tra and Michael R Pisano and Ravi Amunugama and David Allen and Angela K. Walker and John R. Strahler and Phillip C Andrews and Gilbert S. Omenn and Samir M Hanash},
  journal={Proteomics},
  year={2005},
  volume={5 13},
  pages={
          3343-52
        }
}
We have implemented an orthogonal 3-D intact protein analysis system (IPAS) to quantitatively profile protein differences between human serum and plasma. Reference specimens consisting of pooled Caucasian-American serum, citrate-anticoagulated plasma, and EDTA-anticoagulated plasma were each depleted of six highly abundant proteins, concentrated, and labeled with a different Cy dye (Cy5, Cy3, or Cy2). A mixture consisting of each of the labeled samples was subjected to three dimensions of… CONTINUE READING
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