A whole cell biocatalyst for cellulosic ethanol production from dilute acid-pretreated corn stover hydrolyzates

@article{Ryu2011AWC,
  title={A whole cell biocatalyst for cellulosic ethanol production from dilute acid-pretreated corn stover hydrolyzates},
  author={Seunghyun Ryu and M. Nazmul Karim},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={91},
  pages={529-542}
}
In this research, a recombinant whole cell biocatalyst was developed by expressing three cellulases from Clostridium cellulolyticum—endoglucanase (Cel5A), exoglucanase (Cel9E), and β-glucosidase—on the surface of the Escherichia coli LY01. The modified strain is identified as LY01/pRE1H-AEB. The cellulases were displayed on the surface of the cell by fusing with an anchor protein, PgsA. The developed whole cell biocatalyst was used for single-step ethanol fermentation using the phosphoric acid… CONTINUE READING
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Methods for measuring cellulase activities

  • TM Wood, KM Bhat
  • Method Enzymol
  • 1988
Highly Influential
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