A well-mixed, polymer-based microbioreactor with integrated optical measurements.

  title={A well-mixed, polymer-based microbioreactor with integrated optical measurements.},
  author={Zhiyu Zhang and Nicolas Szita and Paolo Boccazzi and Anthony J Sinskey and Klavs F Jensen},
  journal={Biotechnology and bioengineering},
  volume={93 2},
We describe a 150 microL microbioreactor fabricated in poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) to cultivate microbial cell cultures. Mixing is achieved by a small magnetic stir bar and fluorescent sensors are integrated for on-line measurement of pH and dissolved oxygen. Optical transmission measurements are used for cell density. The body of the reactor is poly(methylmethacrylate) with a thin layer of poly (dimethylsiloxane) for aeration, oxygen diffuses through this… CONTINUE READING
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