A volume-sensitive chloride conductance in human colonic cell line T84.

@article{Worrell1989AVC,
  title={A volume-sensitive chloride conductance in human colonic cell line T84.},
  author={Roger T. Worrell and A. Grant Butt and William H. Cliff and Raymond A Frizzell},
  journal={The American journal of physiology},
  year={1989},
  volume={256 6 Pt 1},
  pages={C1111-9}
}
The chloride-secreting colonic cell line, T84, was studied under whole cell patch clamp with Cl as the permeant ion in pipette and bath solutions. Transmembrane current was initially small (approximately 50 pA at +100 mV) but increased steadily to average values of 1-3 nA within 5-10 min. The development of this current was associated with visible cell swelling, either without a shape change or with membrane blebbing. Basal, preswelling current levels were restored by the addition of 50-75 mM… CONTINUE READING