A virus mutant with an insertion of a copia-like transposable element

@article{Miller1982AVM,
  title={A virus mutant with an insertion of a copia-like transposable element},
  author={David W. Miller and Lois K. Miller},
  journal={Nature},
  year={1982},
  volume={299},
  pages={562-564}
}
The important role insect viruses have in the biological control of pests has inspired a considerable effort towards understanding their molecular biology. The nuclear polyhedrosis viruses (NPVs) are particularly interesting and accessible owing to their unique nuclear replication cycle and large (∼100 kilobase, kb) double-stranded, circular DNA genome. Serial passage of NPVs in cell culture results in a gradual reduction in the ability of the virus to carry out the biphasic growth cycle of… Expand
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The data obtained were applied to a model of NPV population genetics in order to derive the frequency distribution of the multiplicity of cell infection in infected insects and estimated that 4.3 baculovirus genomes per OB-producing cell would account for this equilibrium. Expand
An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors
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The initial experimental advances the laboratory has made in developing the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) as a vector in insect cells are described herein. Expand
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Acquisition of Host Cell DNA Sequences by Baculoviruses: Relationship Between Host DNA Insertions and FP Mutants of Autographa californica and Galleria mellonella Nuclear Polyhedrosis Viruses
TLDR
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TLDR
Baculovirus mutagenesis by transposons may increase the genetic heterogeneity and could foster the flow of genetic information from a host genome into a virus genome, and possible consequences of transposon-mutagenesis on genetically engineered bacULoviruses will be discussed. Expand
Characterization of the DA26 gene in a hypervariable region of the Autographa californica nuclear polyhedrosis virus genome.
TLDR
A region of the baculovirus Autographa californica nuclear polyhedrosis virus genome that is frequently found to be altered after serial passage of the virus in cell culture was characterized and the DA26 gene was disrupted both by deletion and by insertion of sequences that resembled transposable elements. Expand
The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells.
TLDR
The baculovirus system has been recognized as a very efficient and safe eukaryotic expression vector and will continue to serve as a model for research on the molecular biology of insects. Expand
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